15674 Adck1 Status: ES cell colonies screened / QC no positives
| Gene | Adck1 |
| Chromosome | 12 |
| Deletion Start | 88,371,774 |
| Deletion End | 88,368,349 |
| Deletion Size | 3,426 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,368,379 | 88,368,397 | ACCAGCGTGGCTCTTGCTG |
| TUP | 88,368,400 | 88,368,429 | TCCGGTGTCTACCTCTACAGTAACAACTAC |
| TUR | 88,368,431 | 88,368,451 | CGCCAAAGTCATTAGGGTCCA |
| TDF | 88,371,002 | 88,371,022 | TCCATTCTCTGATGGCCTCTG |
| TDP | 88,371,024 | 88,371,049 | TCCTGGTTATCCTGGTTCGACACTCA |
| TDR | 88,371,076 | 88,371,095 | TCCACCTGTAAGTGCTGACC |
PCR Primers
| Primer | Sequence |
| SU | CAACAGTGAGCACCCTTTCC |
| SD | CTCTAGAGGCAGCCTACA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
