15690 Tfap2a Status: Cancelled
| Gene | Tfap2a |
| Chromosome | 13 |
| Deletion Start | 40,733,711 |
| Deletion End | 40,717,027 |
| Deletion Size | 16,685 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,733,138 | 40,733,157 | TTCGCCCTGACCTGTAAAGC |
| TUP | 40,733,116 | 40,733,137 | ACGTTCGGCTTGGGAGCCCACA |
| TUR | 40,733,045 | 40,733,062 | AAGCTCGGCGGGAGAAAC |
| TDF | 40,717,478 | 40,717,496 | GCTGGATGGCTGAGGTAAC |
| TDP | 40,717,450 | 40,717,475 | CAGGGAGCGATGGTTTCTCACTACCA |
| TDR | 40,717,406 | 40,717,426 | GCAGTGGAACAGAGACAGAAG |
PCR Primers
| Primer | Sequence |
| SU | AAGAGTTGTTGGGCTTGCAG |
| SD | CTGAAGTACGCAAGGGAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
