15695 Gprin1 Status: Cancelled
| Gene | Gprin1 |
| Chromosome | 13 |
| Deletion Start | 54,740,456 |
| Deletion End | 54,737,631 |
| Deletion Size | 2,826 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,740,293 | 54,740,313 | AGCCTGCCCTTCTCCTAACTG |
| TUP | 54,740,269 | 54,740,292 | CAGCCCTCTCCCAGAGACACCGAG |
| TUR | 54,740,206 | 54,740,226 | ATGGGCTCTGGCTTCCCAAAC |
| TDF | 54,738,491 | 54,738,511 | ATGGCACACCCTACAGCTCAG |
| TDP | 54,738,459 | 54,738,484 | CCCAGAGAGATACCAGGAGCATCGGA |
| TDR | 54,738,403 | 54,738,420 | GCTGCCGCTAGGTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | TGAGATGTGATGAAGCAGGC |
| SD | GAAGCCTCAAACCACCAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
