| Gene | Tert |
| Chromosome | 13 |
| Deletion Start | 73,649,112 |
| Deletion End | 73,627,033 |
| Deletion Size | 22,080 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,627,686 | 73,627,707 | GCCCTCTGTGTCCGCTAGTTAC |
| TUP | 73,627,714 | 73,627,737 | ACCCGACCCGTGGGCAGGAATTTC |
| TUR | 73,627,764 | 73,627,783 | CCTGGCGACTACTGCTCTTG |
| TDF | 73,648,548 | 73,648,570 | CCATAGCTTCCTTCTCCTCCTTC |
| TDP | 73,648,572 | 73,648,599 | TGCTCCCTCTGTTCTGACTCTTATGTCA |
| TDR | 73,648,628 | 73,648,647 | GACCAGGCAGGGATCTTGAG |
PCR Primers
| Primer | Sequence |
| SU | CCTCCGCCTACCTAACCTTC |
| SD | CATGAATTCAGAGTATATTCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15706A-B4 | VGB6 | C57BL/6NTac | |
| 15706A-B10 | VGB6 | C57BL/6NTac | |
| 15706A-C4 | VGB6 | C57BL/6NTac | |
| 15706A-C6 | VGB6 | C57BL/6NTac | |
| 15706A-E1 | VGB6 | C57BL/6NTac | |
| 15706A-H5 | VGB6 | C57BL/6NTac | |
