15711 Tnpo1 Status: Cancelled
| Gene | Tnpo1 |
| Chromosome | 13 |
| Deletion Start | 98,890,778 |
| Deletion End | 98,863,713 |
| Deletion Size | 27,066 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,890,185 | 98,890,205 | GCTGGTGTAGAGGTCCTGTTG |
| TUP | 98,890,155 | 98,890,183 | CACAGTTGGAGAAACGAACATTTGCAGGG |
| TUR | 98,890,104 | 98,890,123 | GCCATCTGCGGGTCTGTTTG |
| TDF | 98,864,050 | 98,864,073 | GCATGTGTCATAGACTCCATTGTC |
| TDP | 98,864,023 | 98,864,046 | TTCACAGGGACCTGGTGACTGCAG |
| TDR | 98,863,950 | 98,863,970 | GCCGGAACATGATATGGGTTG |
PCR Primers
| Primer | Sequence |
| SU | TTGGTCTGCGTGTGCTCCG |
| SD | ACAACAGGACAGGAGACATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 124 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
