15715 Skiv2l2 Status: ES cell colonies screened / QC no positives
| Gene | Skiv2l2 |
| Chromosome | 13 |
| Deletion Start | 112,927,354 |
| Deletion End | 112,901,478 |
| Deletion Size | 25,877 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,926,980 | 112,926,999 | GGCCATGCTTTGGCTGTAAC |
| TUP | 112,926,957 | 112,926,979 | CCCAGCTTAGCAGGCCAGGTACG |
| TUR | 112,926,892 | 112,926,913 | GCCTCGCTGCTTAAAGACTATG |
| TDF | 112,901,555 | 112,901,576 | AGGCTTTATTTGCCACAGAGAC |
| TDP | 112,901,522 | 112,901,551 | TGCTATGGGAATTAACATGCCAGCTAGAAC |
| TDR | 112,901,488 | 112,901,510 | TCCGTCATATTTACGGGCATTTG |
PCR Primers
| Primer | Sequence |
| SU | GAGAGCTCTTCACGTTTGCC |
| SD | GCAGTAAGCACTCTTACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
