15722 Ndst2 Status: Cancelled
| Gene | Ndst2 |
| Chromosome | 14 |
| Deletion Start | 20,730,167 |
| Deletion End | 20,724,179 |
| Deletion Size | 5,989 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,729,459 | 20,729,481 | CCAGTCCAATCACAGCACTTATG |
| TUP | 20,729,437 | 20,729,458 | AGCCCGTGCTTATTGCCAGCCA |
| TUR | 20,729,403 | 20,729,423 | AACTGGTCCTGGCATGGAGAG |
| TDF | 20,724,588 | 20,724,609 | TGAGTACCCTTCTTGCTGACAC |
| TDP | 20,724,563 | 20,724,586 | TGACGGCCTGCTCCTTTGAGTAGG |
| TDR | 20,724,505 | 20,724,526 | GACAGCGAGTCTTACCACCTTC |
PCR Primers
| Primer | Sequence |
| SU | AGATGGGACAAGGAGAGTGG |
| SD | GGACATTTCTAGCCCACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
