| Gene | 5730469M10Rik |
| Chromosome | 14 |
| Deletion Start | 41,004,182 |
| Deletion End | 40,994,489 |
| Deletion Size | 9,694 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,003,388 | 41,003,405 | CCCTGCCAGGTGCATTTC |
| TUP | 41,003,366 | 41,003,387 | CACAGCAGAGCCCAGGCTCAGC |
| TUR | 41,003,314 | 41,003,334 | GCCAGAGGTTCATCCAATCAG |
| TDF | 40,994,697 | 40,994,715 | ACCGCTGGTGCTCAGAATC |
| TDP | 40,994,673 | 40,994,696 | TGGTTCCTGAGTGAACACCTCCGA |
| TDR | 40,994,621 | 40,994,643 | TGCTCAAGAAGGATGCCCTAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGCCATTCCTCTCATTCCTC |
| SD | TTCACTTCAAATACCGGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15730A-A10 | VGB6 | C57BL/6NTac | |
| 15730A-C9 | VGB6 | C57BL/6NTac | |
| 15730A-G11 | VGB6 | C57BL/6NTac | |
| 15730A-H5 | VGB6 | C57BL/6NTac | |
| 15730A-H8 | VGB6 | C57BL/6NTac | |
