15740 Chrna2 Status: Cancelled
| Gene | Chrna2 |
| Chromosome | 14 |
| Deletion Start | 66,151,125 |
| Deletion End | 66,142,217 |
| Deletion Size | 8,909 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,142,471 | 66,142,492 | GCAGCTTTGTTGCCTTAGAATG |
| TUP | 66,142,494 | 66,142,519 | CCCAAGCTTCAGAGTTCCTGACCTCA |
| TUR | 66,142,525 | 66,142,548 | TTGAGTGCTCTATGATGCTGTTTG |
| TDF | 66,150,519 | 66,150,538 | TTGGCCTGTGAGCTGAAGAG |
| TDP | 66,150,541 | 66,150,566 | ACAAAGCTCCAGCTCTTCCACACTGG |
| TDR | 66,150,581 | 66,150,601 | GGGACAGAAAGAGGCAGGTTG |
PCR Primers
| Primer | Sequence |
| SU | CCTTGTCCAATGTGGTTAG |
| SD | TGGGCCAGTGTTCTATTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
