15772 Apobec3 Status: ES cell colonies screened / QC no positives
| Gene | Apobec3 |
| Chromosome | 15 |
| Deletion Start | 79,907,625 |
| Deletion End | 79,892,520 |
| Deletion Size | 15,106 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,892,731 | 79,892,749 | TCTGAGCCGCTTCGGTTAC |
| TUP | 79,892,753 | 79,892,779 | ATAGCTGCTTCTAAACTGGCCACCCTG |
| TUR | 79,892,787 | 79,892,810 | GGAAGAAATATGGGTGACGTGGAG |
| TDF | 79,907,498 | 79,907,520 | ACAGAGTCTCACTGGGTAGCTTG |
| TDP | 79,907,527 | 79,907,554 | TGGAACTCACTACTAGACTAAGCTGGCC |
| TDR | 79,907,577 | 79,907,596 | CTGGCTCTCAGAAGGCACTG |
PCR Primers
| Primer | Sequence |
| SU | TAGGCAGGTTGGATGGAGAC |
| SD | GCCTGCCTGGTTGTCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
