15782 Abcd2 Status: Cancelled
| Gene | Abcd2 |
| Chromosome | 15 |
| Deletion Start | 91,191,605 |
| Deletion End | 91,163,058 |
| Deletion Size | 28,548 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,191,071 | 91,191,094 | CATTGGCCTTTAGAACTCGCTTAG |
| TUP | 91,191,042 | 91,191,069 | AGACCATGCCTATGAGACCTATTTCGCA |
| TUR | 91,190,999 | 91,191,023 | CCAGCCTCCCATCCATATTTATCAC |
| TDF | 91,163,165 | 91,163,184 | TGGAAGTCTCCGGGATCAAG |
| TDP | 91,163,135 | 91,163,160 | TTACCCTGACTCAGCGGATGACATGC |
| TDR | 91,163,093 | 91,163,111 | GCAGGATGCGTTCTAGGTC |
PCR Primers
| Primer | Sequence |
| SU | CTCATTCGACCTCTCCAG |
| SD | GTCTTTAGATGCCAGATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
