| Gene | Krt73 |
| Chromosome | 15 |
| Deletion Start | 101,802,294 |
| Deletion End | 101,793,708 |
| Deletion Size | 8,587 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,801,585 | 101,801,606 | GAGAAGTGAGGCAGAACCAGAG |
| TUP | 101,801,558 | 101,801,583 | AGGCAGAGGAGTCCAGCAGTAGCCTT |
| TUR | 101,801,505 | 101,801,525 | AGAGGGCTCGTGGAAATACAG |
| TDF | 101,794,123 | 101,794,142 | TGGATGCTGGAAGGGACTTG |
| TDP | 101,794,087 | 101,794,113 | CTACCTTGAAGCACTCACTGATGCCCA |
| TDR | 101,794,040 | 101,794,061 | CAGGTGCGGAACTACTGATGAC |
PCR Primers
| Primer | Sequence |
| SU | CCATGACAAGCTTCCTAGCC |
| SD | AGCAAGGTCCAGAATGGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15789A-A3 | VGB6 | C57BL/6NTac | |
| 15789A-G10 | VGB6 | C57BL/6NTac | |
| 15789A-A10 | VGB6 | C57BL/6NTac | |
