15794 Crebbp Status: ES cell colonies screened / QC no positives
| Gene | Crebbp |
| Chromosome | 16 |
| Deletion Start | 4,213,220 |
| Deletion End | 4,179,429 |
| Deletion Size | 33,792 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,212,436 | 4,212,458 | ACCCAAGACTTCCCTGTTCATTC |
| TUP | 4,212,394 | 4,212,421 | CTTCTGGACAGCTCTGATAACCTTGCCA |
| TUR | 4,212,331 | 4,212,350 | TGTTCACAGCAGCATACGAG |
| TDF | 4,179,714 | 4,179,734 | CCAGTAGTCCTGCCACATCAC |
| TDP | 4,179,686 | 4,179,711 | ACTGGACCTGGGATCTGCATGAATGC |
| TDR | 4,179,663 | 4,179,682 | CTGGGTGGGTCTGGTTGAAG |
PCR Primers
| Primer | Sequence |
| SU | GCGGGCGAATTCATTAAG |
| SD | CTACATAGTTTCCTCGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
