| Gene | Sept12 |
| Chromosome | 16 |
| Deletion Start | 4,996,600 |
| Deletion End | 4,988,258 |
| Deletion Size | 8,343 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,996,513 | 4,996,532 | GTTTGGTCCTGTGGGCATTG |
| TUP | 4,996,487 | 4,996,512 | AGGCTGTGCTAGACCAGCTGAGGATC |
| TUR | 4,996,428 | 4,996,450 | TCTGCCTCTGACTCACCTACTAC |
| TDF | 4,988,517 | 4,988,541 | GAGAGCACATAGTGAATGGGAGATG |
| TDP | 4,988,494 | 4,988,516 | TGTCCTGGGCCGGAAGACCAAAT |
| TDR | 4,988,456 | 4,988,474 | GCCTGCAATGTTCCCAAAC |
PCR Primers
| Primer | Sequence |
| SU | TTGGGACAGGATGGACTG |
| SD | TGCCGTGGCTCTCCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15797A-A6 | VGB6 | C57BL/6NTac | |
| 15797A-B6 | VGB6 | C57BL/6NTac | |
| 15797A-C12 | VGB6 | C57BL/6NTac | |
| 15797A-E9 | VGB6 | C57BL/6NTac | |
| 15797A-H5 | VGB6 | C57BL/6NTac | |
| 15797A-H7 | VGB6 | C57BL/6NTac | |
