| Gene | Fgd4 |
| Chromosome | 16 |
| Deletion Start | 16,490,511 |
| Deletion End | 16,461,598 |
| Deletion Size | 28,914 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,490,151 | 16,490,171 | TGTGTGCCATCCCTATCTCTG |
| TUP | 16,490,125 | 16,490,148 | TTCCTCACTCTCTGAGGGCCTGAC |
| TUR | 16,490,096 | 16,490,116 | GAGGCTTAGGAGGGCAAGAAG |
| TDF | 16,462,178 | 16,462,202 | GCCATGAACAAATGACTTAGGAAAC |
| TDP | 16,462,144 | 16,462,169 | AGACTGTAGTGGGAACTGCTGTGACC |
| TDR | 16,462,090 | 16,462,112 | TTGTCCTTCCTGTGCTACTAGAG |
PCR Primers
| Primer | Sequence |
| SU | GAGATCTGGAGAAATAGCAAGAGC |
| SD | CTATAAGGACACAAGGAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15804A-H12 | VGB6 | C57BL/6NTac | |
| 15804A-H3 | VGB6 | C57BL/6NTac | |
| 15804A-F11 | VGB6 | C57BL/6NTac | |
| 15804A-C4 | VGB6 | C57BL/6NTac | |
| 15804A-E9 | VGB6 | C57BL/6NTac | |
