| Gene | Top3b |
| Chromosome | 16 |
| Deletion Start | 16,892,978 |
| Deletion End | 16,877,950 |
| Deletion Size | 15,029 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,878,685 | 16,878,708 | AGAAATGGAGTGAGACCTGAAGAG |
| TUP | 16,878,709 | 16,878,734 | TCTCCGCCTCTAGTTCCCAGGAAGTA |
| TUR | 16,878,773 | 16,878,793 | AATGGTGCTGAGGGATGGAAG |
| TDF | 16,892,348 | 16,892,369 | GCATTGAAAGCAGCCAGAGTTG |
| TDP | 16,892,378 | 16,892,402 | AGGGCATGCTTAGGTCACTGGCCTC |
| TDR | 16,892,408 | 16,892,425 | GCAGCCCATGCCTGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGAGTGCGTAACCCAGTGAG |
| SD | ATGTCACCAGAGTCACACGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15805A-D6 | VGB6 | C57BL/6NTac | |
| 15805A-G1 | VGB6 | C57BL/6NTac | |
| 15805A-G11 | VGB6 | C57BL/6NTac | |
