15811 Lpp Status: Cancelled
| Gene | Lpp |
| Chromosome | 16 |
| Deletion Start | 24,608,313 |
| Deletion End | 24,608,128 |
| Deletion Size | 186 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,608,153 | 24,608,174 | AAAGCACTGGTGAGCCTCTTGG |
| TUP | 24,608,175 | 24,608,196 | CCACGTGCCTGCACGGATGGAG |
| TUR | 24,608,234 | 24,608,253 | CTTGGGTGGCTGTTGTGTTG |
| TDF | 24,608,184 | 24,608,201 | TGCACGGATGGAGACCAC |
| TDP | 24,608,218 | 24,608,246 | CCCAGTATCTCAGTGTCAACACAACAGCC |
| TDR | 24,608,264 | 24,608,283 | GGGTTTCGGAGCAACAACTG |
PCR Primers
| Primer | Sequence |
| SU | TTTCCCTGATTTGCAGGTTC |
| SD | AAAGGTTCTGAGTCACACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
