15820 Drd3 Status: Cancelled
| Gene | Drd3 |
| Chromosome | 16 |
| Deletion Start | 43,790,706 |
| Deletion End | 43,762,245 |
| Deletion Size | 28,462 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,762,960 | 43,762,983 | CTCTTCCTCGTGTTTCAACTACTC |
| TUP | 43,762,986 | 43,763,011 | CATCTTGGCTTTGTCCTCCTTGGTGC |
| TUR | 43,763,017 | 43,763,039 | CTTTGGCAGCAGAATCTATGGAG |
| TDF | 43,790,394 | 43,790,418 | CCTTCCTTAGCAATCAATCCCAGAC |
| TDP | 43,790,419 | 43,790,442 | AGCCTGGCCTACCCTCTGTGCCTG |
| TDR | 43,790,457 | 43,790,474 | TGTGGTAGGTGCCCTCAG |
PCR Primers
| Primer | Sequence |
| SU | CCTCTGCTAGCCTTGACTTCAC |
| SD | CCCTTGCCTTCCAACTAGAA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
