15846 Wdr46 Status: Cancelled
| Gene | Wdr46 |
| Chromosome | 17 |
| Deletion Start | 33,949,589 |
| Deletion End | 33,940,741 |
| Deletion Size | 8,849 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,941,399 | 33,941,416 | GCCGTAGCTCCCACATTC |
| TUP | 33,941,428 | 33,941,456 | CTCAAAGCAAACTCGAGAAGGCTGAAGCG |
| TUR | 33,941,460 | 33,941,477 | GACGCTTGCCTCTTCCTC |
| TDF | 33,948,645 | 33,948,666 | GCTGCGGAGCCTAACTTTGATG |
| TDP | 33,948,669 | 33,948,694 | TTGGAGAATAACCCATACCGGAGCCG |
| TDR | 33,948,708 | 33,948,726 | GGGCCTTCACCTCCCATTC |
PCR Primers
| Primer | Sequence |
| SU | TCTGACTTGTGTGAGGCTCG |
| SD | TTGGTCTTTGCGACAACCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
