| Gene | Ubd |
| Chromosome | 17 |
| Deletion Start | 37,195,737 |
| Deletion End | 37,193,955 |
| Deletion Size | 1,783 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,194,201 | 37,194,224 | GTGCATGTATATGGTGGGTCAGAG |
| TUP | 37,194,236 | 37,194,265 | CCTTCAGGAGTCAGTTCTCTCTCTTCACCA |
| TUR | 37,194,293 | 37,194,310 | CTTGCTGCCAAGCCTCAC |
| TDF | 37,195,199 | 37,195,218 | AGGGTGGCAGGCGTTCTTAG |
| TDP | 37,195,220 | 37,195,246 | TGACGCTCTTGGCTTTCTTTGCCTAGG |
| TDR | 37,195,275 | 37,195,299 | GTCATTCTCAGTGGTCTCAAAGGTC |
PCR Primers
| Primer | Sequence |
| SU | AGGATTGGGAGAAGGTAGGG |
| SD | TTCAATTTGGTTCAACAATCATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15849A-C3 | VGB6 | C57BL/6NTac | |
| 15849A-G2 | VGB6 | C57BL/6NTac | |
| 15849A-D2 | VGB6 | C57BL/6NTac | |
| 15849A-E8 | VGB6 | C57BL/6NTac | |
