15856 Rab5a Status: ES cell colonies screened / QC no positives
| Gene | Rab5a |
| Chromosome | 17 |
| Deletion Start | 53,506,463 |
| Deletion End | 53,483,916 |
| Deletion Size | 22,548 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,484,630 | 53,484,650 | TAGGCTGGCTTGGAACTGAAG |
| TUP | 53,484,659 | 53,484,683 | TTATGGATGACTTGAATATTGGTCT |
| TUR | 53,484,698 | 53,484,718 | TGGCCCACCTGTAATCTCAAC |
| TDF | 53,505,490 | 53,505,513 | GCTTGGATTTTATGTGTGTCTTGC |
| TDP | 53,505,533 | 53,505,556 | TGGAAATCTTGATATGCTACTCGG |
| TDR | 53,505,592 | 53,505,615 | TCAGTACAGAGAAGGAGTTGGAAC |
PCR Primers
| Primer | Sequence |
| SU | CCACTGCTGTTCTTTGTC |
| SD | CATTCCTTCTTTCTGGAGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 126 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
