15887 Isoc1 Status: ES cell colonies screened / QC no positives
| Gene | Isoc1 |
| Chromosome | 18 |
| Deletion Start | 58,677,964 |
| Deletion End | 58,659,495 |
| Deletion Size | 18,470 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,660,103 | 58,660,124 | TGCAGCAGCCACTTAAATAGTC |
| TUP | 58,660,127 | 58,660,152 | TGTTGAAAGCTCCGTGAATCCTAGGC |
| TUR | 58,660,154 | 58,660,178 | TCCAATCCTTAGGTACAACTTCCTC |
| TDF | 58,677,774 | 58,677,793 | CGCACTGGGATCATCGTGAC |
| TDP | 58,677,796 | 58,677,822 | CTAGTGAGGCTGTCCTCCTACAGCTGG |
| TDR | 58,677,828 | 58,677,849 | TGAACTTCGGGTGGTCTTTGTC |
PCR Primers
| Primer | Sequence |
| SU | GTGATGCTCTGTACCTAC |
| SD | ACTGACATGACAAGGGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
