15890 Lman1 Status: Cancelled
| Gene | Lman1 |
| Chromosome | 18 |
| Deletion Start | 66,002,608 |
| Deletion End | 65,983,834 |
| Deletion Size | 18,775 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,002,046 | 66,002,067 | TCTGCGTGTCGTGTTACCTTAC |
| TUP | 66,002,019 | 66,002,044 | CCCTAATGCCACGTCTCATGGGTACA |
| TUR | 66,001,984 | 66,002,007 | TGGAACTGGTCTCAGATTGCTATG |
| TDF | 65,984,486 | 65,984,506 | CCTCCGTGCTGATGACTTGAC |
| TDP | 65,984,458 | 65,984,483 | TGACAAAGAAGAGCAGCCTTATGCCA |
| TDR | 65,984,436 | 65,984,457 | TCTTCCGCTTTCACAGAACAAC |
PCR Primers
| Primer | Sequence |
| SU | CTACAGAGCTGGACACACCG |
| SD | GACACAGAGGGTATCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
