| Gene | Zfpl1 |
| Chromosome | 19 |
| Deletion Start | 6,084,450 |
| Deletion End | 6,081,010 |
| Deletion Size | 3,441 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,083,700 | 6,083,721 | GTCTGGGAGAGCTAGAGATGAC |
| TUP | 6,083,671 | 6,083,695 | AGCTGTGGCTGGGAGAAAGATCCAC |
| TUR | 6,083,623 | 6,083,644 | GGATAGCTTCCTCTGGGTACAG |
| TDF | 6,081,072 | 6,081,092 | CAGCAGACAGTGATCCCAATC |
| TDP | 6,081,050 | 6,081,071 | TGGACCCGCTCATGAACCCTCA |
| TDR | 6,081,025 | 6,081,044 | GGCTCAGGAAGGACCTACAC |
PCR Primers
| Primer | Sequence |
| SU | GGAGTTGGTGAGCTCAGTCC |
| SD | TCCTCAATGGAGCCATGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15902A-B5 | VGB6 | C57BL/6NTac | |
| 15902A-H2 | VGB6 | C57BL/6NTac | |
| 15902A-E3 | VGB6 | C57BL/6NTac | |
| 15902A-H11 | VGB6 | C57BL/6NTac | |
| 15902A-E2 | VGB6 | C57BL/6NTac | |
