| Gene | Rln1 |
| Chromosome | 19 |
| Deletion Start | 29,334,593 |
| Deletion End | 29,331,763 |
| Deletion Size | 2,831 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,334,179 | 29,334,200 | CAGGCAAGGGCTTCATTTACAC |
| TUP | 29,334,148 | 29,334,173 | ATGGAGCTAGGACCTAACGGGAGCCA |
| TUR | 29,334,094 | 29,334,118 | GTCTTACAAACAGGGAGCAGTAGTC |
| TDF | 29,332,029 | 29,332,047 | GAGCTACAACACGCACCTG |
| TDP | 29,332,001 | 29,332,028 | TGTTGAGCGATTCTGTTGTTAGCTTGGA |
| TDR | 29,331,950 | 29,331,968 | CTGCCGTCTTCTGCTTCAC |
PCR Primers
| Primer | Sequence |
| SU | ATTGCTGATGTGACTTAGTCC |
| SD | AGAGAGCATTGTATTAGCATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15917A-B5 | VGB6 | C57BL/6NTac | |
| 15917A-G8 | VGB6 | C57BL/6NTac | |
| 15917A-C8 | VGB6 | C57BL/6NTac | |
