| Gene | Cox15 |
| Chromosome | 19 |
| Deletion Start | 43,752,908 |
| Deletion End | 43,736,696 |
| Deletion Size | 16,213 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,752,713 | 43,752,734 | GCTCGCCTTCTCATTTCAGGAC |
| TUP | 43,752,686 | 43,752,709 | TTTCCTTGTGTCCCGACACCTCCC |
| TUR | 43,752,635 | 43,752,658 | AAATTGGTCAGTCATACACAGCAG |
| TDF | 43,737,564 | 43,737,586 | TGGAAACAAACTAAAGCCACCAC |
| TDP | 43,737,539 | 43,737,563 | CGGATGGCTCTGCTGCAAGATGCAG |
| TDR | 43,737,510 | 43,737,533 | TGTCCCTCGCTCTTTCTTAAACTG |
PCR Primers
| Primer | Sequence |
| SU | ACGATATCCACGCCTGTTTC |
| SD | TGTCACTGTCACCAGAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15926A-D3 | VGB6 | C57BL/6NTac | |
| 15926A-G9 | VGB6 | C57BL/6NTac | |
