| Gene | Atp6ap1 |
| Chromosome | X |
| Deletion Start | 74,304,010 |
| Deletion End | 74,297,182 |
| Deletion Size | 6,829 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | AGCCAGGAGCCGATAGGGTTAG |
| TUP | | | TCAAGCAACGAAATCAGGTCTGACAGTCC |
| TUR | | | CCACAGGAGCCCACAGATTC |
| TDF | | | CCCAAGCTGGTGGCAGTAAG |
| TDP | | | AGGGAAGAAGGGCCCAGAGAATCTCA |
| TDR | | | CTGCACAAGGCTGGAACTCTC |
PCR Primers
| Primer | Sequence |
| SU | AGACTGTCCTCCATCCTGCC |
| SD | TCGCAAATTCAAGGAACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15936A-D4 | VGB6 | C57BL/6NTac | |
| 15936A-H7 | VGB6 | C57BL/6NTac | |
