15965 Egr2 Status: Cancelled
| Gene | Egr2 |
| Chromosome | 10 |
| Deletion Start | 67,541,004 |
| Deletion End | 67,538,374 |
| Deletion Size | 2,631 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,538,629 | 67,538,647 | GCTAGGCGCAGGGTTGAAG |
| TUP | 67,538,650 | 67,538,676 | TCATCTCCTGGTCCTTGAAGCAGGTCC |
| TUR | 67,538,707 | 67,538,731 | TGATGAACTTTCCCATCTCTTTGAC |
| TDF | 67,540,285 | 67,540,302 | ATCCACACGGTGCTGCTG |
| TDP | 67,540,304 | 67,540,329 | CCCAGATCGAAAGCCGTTTCCCTGTC |
| TDR | 67,540,330 | 67,540,349 | CACTCGCAGGGAGTCCAGAG |
PCR Primers
| Primer | Sequence |
| SU | GGATCACAGGCAGGAGAGAG |
| SD | CAAAGGAATCAGCAACTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
