15971 Nav3 Status: Cancelled
| Gene | Nav3 |
| Chromosome | 10 |
| Deletion Start | 110,000,216 |
| Deletion End | 109,999,981 |
| Deletion Size | 236 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,000,159 | 110,000,177 | GTTGGGCCAAAGCCTGTTC |
| TUP | 110,000,131 | 110,000,157 | CGCTGCTCTTCCGATACCGAATCTTGG |
| TUR | 110,000,112 | 110,000,130 | GCGCCGTGACACACTGATG |
| TDF | 110,000,050 | 110,000,067 | GCTTTCGTGCCAGCTCAC |
| TDP | 110,000,012 | 110,000,038 | CCTGTGAGTTTGGAGAGAAGAAAGCCC |
| TDR | 109,999,984 | 110,000,005 | TCTTCGATCTCCTTGGCTGTTC |
PCR Primers
| Primer | Sequence |
| SU | TCTGAACTAGCAAGACAGATTCTC |
| SD | ATAGAGGACACGCACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
