15972 Clint1 Status: Cancelled
| Gene | Clint1 |
| Chromosome | 11 |
| Deletion Start | 45,883,810 |
| Deletion End | 45,852,155 |
| Deletion Size | 31,656 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,852,648 | 45,852,667 | CTGTGGACAGGCTGGAGTTG |
| TUP | 45,852,670 | 45,852,692 | CAAGAGGAGACTCGCCCTTCGGG |
| TUR | 45,852,715 | 45,852,736 | CACGGAAAGTTGTCTGGTCATC |
| TDF | 45,883,422 | 45,883,442 | GAGCGTGTCCGTTTGTCTTTG |
| TDP | 45,883,446 | 45,883,471 | TCAGACCATGTCTCCTGGACTTGGGC |
| TDR | 45,883,484 | 45,883,507 | AGCTTTCAAGAATGCAGAGGAATG |
PCR Primers
| Primer | Sequence |
| SU | GAGGCTGTGTCAGAGGTC |
| SD | ACGGTGCCATCCTGCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
