15973 Gria1 Status: Cancelled
| Gene | Gria1 |
| Chromosome | 11 |
| Deletion Start | 57,327,643 |
| Deletion End | 57,324,393 |
| Deletion Size | 3,251 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,012,214 | 57,012,238 | GGGAGATTTCTCAACCCATTCTCAG |
| TUP | 57,012,240 | 57,012,264 | CGGAGCTGTTGTGCTTAGCAATGGA |
| TUR | 57,012,272 | 57,012,292 | GACCCAAGTCTTCCTCCCTTC |
| TDF | 57,014,546 | 57,014,566 | GGATGGCAATGGGCAAGTTAC |
| TDP | 57,014,571 | 57,014,593 | CATCCTAGCACGCAGACCTGGCC |
| TDR | 57,014,647 | 57,014,671 | GTGTTGCAGAGTGTTAGAAATGGAG |
PCR Primers
| Primer | Sequence |
| SU | GAGCTGGCTCCTGGGTATC |
| SD | CTCAACTCAGGACCAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 126 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
