16034 Scaf4 Status: Cancelled
| Gene | Scaf4 |
| Chromosome | 16 |
| Deletion Start | 90,284,029 |
| Deletion End | 90,252,261 |
| Deletion Size | 31,769 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,283,378 | 90,283,396 | AGGCTGCTTGGGTCTCCAC |
| TUP | 90,283,354 | 90,283,373 | TCACCCTCGCACCGCCCTCT |
| TUR | 90,283,277 | 90,283,296 | GCAGCTTTGTTCCCGGTTTG |
| TDF | 90,252,411 | 90,252,433 | CGATTACGATGACGAGCCAGAAG |
| TDP | 90,252,380 | 90,252,409 | TGTGGAAGACTCAAAGAAAGAGGATGCTGC |
| TDR | 90,252,358 | 90,252,376 | AAGGGCTGCGGTGCTGATG |
PCR Primers
| Primer | Sequence |
| SU | GTGTCAGCGCGACGCAGA |
| SD | GGTCCATACTTTGATGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
