16040 Zfp40 Status: ES cell colonies screened / QC no positives
| Gene | Zfp40 |
| Chromosome | 17 |
| Deletion Start | 23,191,376 |
| Deletion End | 23,174,441 |
| Deletion Size | 16,936 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,191,133 | 23,191,155 | TTGACGTTGCATGTGAGTAATGG |
| TUP | 23,191,105 | 23,191,130 | TCCCTCTCCTAGTCACAGCTCTGGTT |
| TUR | 23,191,031 | 23,191,055 | GGCATCAATGGTTCTAACTAAACAC |
| TDF | 23,174,818 | 23,174,841 | GGCAGGAAATAAACTCAAGACATC |
| TDP | 23,174,791 | 23,174,816 | TCAGGCAGTACCTGAAACTGACAGGA |
| TDR | 23,174,740 | 23,174,764 | TCTTCTAAGCAGCAAAACTGGTATG |
PCR Primers
| Primer | Sequence |
| SU | GTTCTTCCTCAAGGTGCTGG |
| SD | AAGCATGAGCTCAGAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
