16042 Fkbp5 Status: Cancelled
| Gene | Fkbp5 |
| Chromosome | 17 |
| Deletion Start | 28,441,029 |
| Deletion End | 28,410,514 |
| Deletion Size | 30,516 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,440,783 | 28,440,805 | GCTTCATAGCTGGGCGTAATCTC |
| TUP | 28,440,753 | 28,440,780 | ATTATAAATGGTGCAGCTTCCGCTGGGC |
| TUR | 28,440,696 | 28,440,717 | GGGAACGCATAACTGGAACTTG |
| TDF | 28,411,474 | 28,411,492 | AACTTGCCGTGGCCTTCAG |
| TDP | 28,411,450 | 28,411,472 | CTGGTGTACCTCCTCGGACAGCC |
| TDR | 28,411,427 | 28,411,444 | TGCAAAGACAGCCCAGAG |
PCR Primers
| Primer | Sequence |
| SU | TATGGAAAGGGAGCTGGTTG |
| SD | GATCATCCAAAGTGAACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
