16043 Glp1r Status: ES cell colonies screened / QC no positives
| Gene | Glp1r |
| Chromosome | 17 |
| Deletion Start | 30,936,448 |
| Deletion End | 30,901,880 |
| Deletion Size | 34,569 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,902,632 | 30,902,655 | ACCAGAAAGAATACCGAGGGTCAC |
| TUP | 30,902,660 | 30,902,684 | ACATCCAACCTCCTTGGTGTCGCCA |
| TUR | 30,902,705 | 30,902,725 | TCAGAGAAGAGCCCACAAGAG |
| TDF | 30,935,682 | 30,935,705 | GGAGGAGGGTGAAGAGTTCAAATC |
| TDP | 30,935,711 | 30,935,740 | TAGGCTACCTAGTGAGACTTTGCTGTCAGA |
| TDR | 30,935,748 | 30,935,770 | ACTCGGGTCTGGGTTTATTGTTG |
PCR Primers
| Primer | Sequence |
| SU | TGGCTTTCCTTAGTTGAGGG |
| SD | CTCTGGACTGGCTGAGGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
