16045 Gabbr1 Status: ES cell colonies screened / QC no positives
| Gene | Gabbr1 |
| Chromosome | 17 |
| Deletion Start | 37,073,032 |
| Deletion End | 37,046,698 |
| Deletion Size | 26,335 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,046,898 | 37,046,916 | CGCAGCCACTTCACAACTC |
| TUP | 37,046,918 | 37,046,941 | TGACCTCCTTTCTGTCACTCCGCA |
| TUR | 37,046,962 | 37,046,985 | GTAGTGTAGCAACGTGAGGTAGAG |
| TDF | 37,072,310 | 37,072,331 | CCCTGTCTAAGGGCAGATTGTC |
| TDP | 37,072,335 | 37,072,359 | AGCTGAGCCTCAGGTACAGGTTCAA |
| TDR | 37,072,360 | 37,072,380 | CCCTCCGTAAATCACCAACAC |
PCR Primers
| Primer | Sequence |
| SU | GCTGCTGAGAGGAGGTGG |
| SD | AAGACACAGGGACATGGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
