16048 Ptprs Status: ES cell colonies screened / QC no positives
| Gene | Ptprs |
| Chromosome | 17 |
| Deletion Start | 56,458,279 |
| Deletion End | 56,428,856 |
| Deletion Size | 29,424 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,458,061 | 56,458,082 | TGCTCAAGGAAGTGGTTTGGTG |
| TUP | 56,458,032 | 56,458,058 | TAGCTTTGGGAAAGGCTGTAGACCACA |
| TUR | 56,458,010 | 56,458,028 | ACTGTCCAGCCAGCATCAC |
| TDF | 56,429,091 | 56,429,112 | CCACGGCCATATTGGTAAGTTG |
| TDP | 56,429,066 | 56,429,087 | CCCGCCACCGCCAGAAACTCAC |
| TDR | 56,429,030 | 56,429,048 | TCGGTAGCGGACGCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TCAGAAGGTCAAGGTCCAGG |
| SD | CTGACACTAACAGCACTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
