16049 2610034M16Rik Status: ES cell colonies screened / QC no positives
| Gene | 2610034M16Rik |
| Chromosome | 17 |
| Deletion Start | 58,987,469 |
| Deletion End | 58,967,318 |
| Deletion Size | 20,152 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,987,038 | 58,987,058 | CCGGCATGGTGAAAAGGAAAG |
| TUP | 58,987,008 | 58,987,034 | AGCTCCTCTGTGCATCATTTCCTTGGA |
| TUR | 58,986,932 | 58,986,953 | AAGTGCCCATCATGTGTTTGAG |
| TDF | 58,967,452 | 58,967,471 | TCCCAGGAACTGGCCCTAAG |
| TDP | 58,967,424 | 58,967,448 | TGGAAGAGCCAGTGATGGTTCCCAG |
| TDR | 58,967,401 | 58,967,420 | GCCCACTGCTCTATTGCTTC |
PCR Primers
| Primer | Sequence |
| SU | GAACATTCACAGAGCTGAGGG |
| SD | GCTGGTAAGTAACACACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
