16051 Myom1 Status: ES cell colonies screened / QC no positives
| Gene | Myom1 |
| Chromosome | 17 |
| Deletion Start | 71,052,771 |
| Deletion End | 71,022,878 |
| Deletion Size | 29,894 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,023,129 | 71,023,148 | CCGGCATTCTCAAGGGTCTG |
| TUP | 71,023,152 | 71,023,173 | TCAGCCGGAAGACAGCCTCTGC |
| TUR | 71,023,198 | 71,023,217 | GAGGGCTGCTCCTGACATAC |
| TDF | 71,052,687 | 71,052,711 | CCTCAACAAAGAAGATGAAGGTCTC |
| TDP | 71,052,712 | 71,052,735 | TACACGATCCGAGTGCGAATGGGA |
| TDR | 71,052,744 | 71,052,765 | GACGTAAGCACTGTACTGTTCG |
PCR Primers
| Primer | Sequence |
| SU | AGGTGACTCTGTTTGGCAGC |
| SD | CTTCATCAACCCAAAGTCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
