16052 Slc30a6 Status: ES cell colonies screened / QC no positives
| Gene | Slc30a6 |
| Chromosome | 17 |
| Deletion Start | 74,423,544 |
| Deletion End | 74,395,669 |
| Deletion Size | 27,876 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,395,802 | 74,395,822 | TCTTGAAGGGTTCCTGCTCTG |
| TUP | 74,395,823 | 74,395,847 | TGCCTCTACAGCTGGGAAATGCCAG |
| TUR | 74,395,885 | 74,395,903 | CGCTGATGGCGTTCACTTG |
| TDF | 74,423,143 | 74,423,161 | CGGCCTGCCTTATCCTCTG |
| TDP | 74,423,164 | 74,423,187 | CCTGTTGCGCCCAACGTCCTAAAC |
| TDR | 74,423,194 | 74,423,215 | GCATTGGGATTACGTGATGGTC |
PCR Primers
| Primer | Sequence |
| SU | GACCTCGAAATCAGCGAGAC |
| SD | AGTTGGGCACCATACATGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
