| Gene | Armc3 |
| Chromosome | 2 |
| Deletion Start | 19,235,594 |
| Deletion End | 19,201,771 |
| Deletion Size | 33,824 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,202,195 | 19,202,219 | ATGGTATTTCCTGAGAGTCTTGGAC |
| TUP | 19,202,223 | 19,202,247 | AGATCTCCTGTATTGTATTTTCCAA |
| TUR | 19,202,293 | 19,202,317 | GCAGTAAGAGGCTGTGTTTATGTTC |
| TDF | 19,234,663 | 19,234,685 | TGCAGGAGAAACAAATACCACAC |
| TDP | 19,234,687 | 19,234,712 | AAGTGCCTCTGCTGGCGTTTCTACGG |
| TDR | 19,234,723 | 19,234,746 | GGACCATAGAGCTACACTAGACAG |
PCR Primers
| Primer | Sequence |
| SU | GATACCGCAAGTGCACAAC |
| SD | GAGAATACCCTGAGTGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16065A-C11 | VGB6 | C57BL/6NTac | |
| 16065A-D9 | VGB6 | C57BL/6NTac | |
