16071 Madd Status: Cancelled
| Gene | Madd |
| Chromosome | 2 |
| Deletion Start | 91,179,234 |
| Deletion End | 91,146,843 |
| Deletion Size | 32,392 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,178,335 | 91,178,355 | GATGAGCCTTCGGGACGATAC |
| TUP | 91,178,301 | 91,178,328 | TGTCTTCACCCTAACCGATAAGGACACC |
| TUR | 91,178,280 | 91,178,297 | GATGCCATAGCGGGTGAC |
| TDF | 91,147,365 | 91,147,388 | ACTCCAACAAGTCTTTGGTCCTTC |
| TDP | 91,147,341 | 91,147,364 | CCTGTGGCCAGTGGCTAGCAGATA |
| TDR | 91,147,252 | 91,147,272 | GGCCTCGAACTCAGAGATCAG |
PCR Primers
| Primer | Sequence |
| SU | AGTCTCAGAAACTCGGCTGG |
| SD | GGCTATGGTCACACCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 137 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
