16075 Fermt1 Status: ES cell colonies screened / QC no positives
| Gene | Fermt1 |
| Chromosome | 2 |
| Deletion Start | 132,942,084 |
| Deletion End | 132,906,626 |
| Deletion Size | 35,459 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 132,941,297 | 132,941,314 | CATCACCTGCTCCCTGTC |
| TUP | 132,941,274 | 132,941,295 | CAAGCCCACACTGCTGCCCACT |
| TUR | 132,941,238 | 132,941,259 | AGGATGGTGGTTGCCCTTAGTG |
| TDF | 132,906,912 | 132,906,933 | CCCTCGATAGTTCTCAGCCTTG |
| TDP | 132,906,880 | 132,906,908 | ACCATCTCCAGCTGCTTTCTCATCCTTCC |
| TDR | 132,906,825 | 132,906,844 | TCAAACTCGATTGCCACCTG |
PCR Primers
| Primer | Sequence |
| SU | TTGATATGGGCAAGAAATGC |
| SD | TGTGCCTGCAAAGCCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
