16076 Zfp120 Status: ES cell colonies screened / QC no positives
| Gene | Zfp120 |
| Chromosome | 2 |
| Deletion Start | 150,136,639 |
| Deletion End | 150,116,468 |
| Deletion Size | 20,172 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 150,136,480 | 150,136,498 | TCGTCCCAGGGCTCAGTAG |
| TUP | 150,136,453 | 150,136,478 | AAACAGCAGCCTGACAGAAGCTGAGA |
| TUR | 150,136,432 | 150,136,452 | GACCACAGGGAAGCTCACAAG |
| TDF | 150,116,629 | 150,116,650 | TACTGATAGCTCAGGGCTACAG |
| TDP | 150,116,595 | 150,116,621 | TTGGAAACTGAACTGTGACATGCTGCC |
| TDR | 150,116,577 | 150,116,594 | TGGGTCGGGAAGTGGTTC |
PCR Primers
| Primer | Sequence |
| SU | CCTACTGAGTGTTGTGAC |
| SD | GCCTGTGATATGCAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
