16089 Adh4 Status: Cancelled
| Gene | Adh4 |
| Chromosome | 3 |
| Deletion Start | 138,430,753 |
| Deletion End | 138,415,535 |
| Deletion Size | 15,219 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,415,572 | 138,415,590 | CATTGGGCTGCCGCTTCTG |
| TUP | 138,415,592 | 138,415,619 | TGTGTCTTTGCACATTTGCTGAAGATGC |
| TUR | 138,415,630 | 138,415,652 | AGTCGCTGTGCCTTGAGTGTAAC |
| TDF | 138,430,061 | 138,430,081 | GCTCCTGTCAGATCATTCCAC |
| TDP | 138,430,085 | 138,430,109 | TCCAGCAGTTTCAGCTGCACTGTGC |
| TDR | 138,430,153 | 138,430,174 | AGCCTCTAGGAAGCCCTCATTC |
PCR Primers
| Primer | Sequence |
| SU | TCCAGAATGATGGAGATGACC |
| SD | CCAAACAGAAAACACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
