16110 Yes1 Status: ES cell colonies screened / QC no positives
| Gene | Yes1 |
| Chromosome | 5 |
| Deletion Start | 32,660,899 |
| Deletion End | 32,640,342 |
| Deletion Size | 20,558 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,640,402 | 32,640,421 | ACAGAGCCTGTAAGCCCAAG |
| TUP | 32,640,422 | 32,640,448 | TGCCAGTCATTATGGAGTGGAACATGC |
| TUR | 32,640,499 | 32,640,520 | CCAAAGGGTGTCATGGAAAGAC |
| TDF | 32,659,901 | 32,659,923 | ACAGTTGATGAACTTGCTTCTGC |
| TDP | 32,659,929 | 32,659,953 | TTACCTATCAGTAAGAATCCACATA |
| TDR | 32,659,977 | 32,659,998 | TGCCTGTCTCCTCTGAAGTTAC |
PCR Primers
| Primer | Sequence |
| SU | AGTTTGTATTGGCTGGGAGG |
| SD | CAGATGACAGGGATTCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 119 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
