| Gene | Wbscr17 |
| Chromosome | 5 |
| Deletion Start | 131,306,597 |
| Deletion End | 131,306,368 |
| Deletion Size | 230 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,306,553 | 131,306,576 | GTGTTGTTGGTGTTGAACTTGATC |
| TUP | 131,306,529 | 131,306,551 | CGGTGGCCGGCTTCGTGATCTTT |
| TUR | 131,306,505 | 131,306,524 | CGCGATAGGCCGACACTTAG |
| TDF | 131,306,446 | 131,306,464 | CAGAGGTGGCCAACCTCAG |
| TDP | 131,306,425 | 131,306,443 | CGCACAGCGCCAGCCCCAT |
| TDR | 131,306,368 | 131,306,387 | AGCTGCCTGTAGACGATGTC |
PCR Primers
| Primer | Sequence |
| SU | CAAAGTCTAAAGGCAACCTG |
| SD | TTTACTCGAGCCCTCACT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16112A-B3 | VGB6 | C57BL/6NTac | |
| 16112A-H5 | VGB6 | C57BL/6NTac | |
| 16112A-F1 | VGB6 | C57BL/6NTac | |
| 16112A-G8 | VGB6 | C57BL/6NTac | |
