16115 Rab7 Status: Cancelled
| Gene | Rab7 |
| Chromosome | 6 |
| Deletion Start | 88,013,687 |
| Deletion End | 88,000,384 |
| Deletion Size | 13,304 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,013,245 | 88,013,263 | GGCAAGGCAAGCACTACTG |
| TUP | 88,013,212 | 88,013,240 | AGGCTGTATGTATGTGCACTCTTTGTGGA |
| TUR | 88,013,170 | 88,013,192 | GCAACCTAAAGAAAGCGCCAATG |
| TDF | 88,000,883 | 88,000,902 | ACGGCATCACGGTAGCTCTG |
| TDP | 88,000,853 | 88,000,880 | CCTTCAGCTGCTATGTGGAAACAAAGGC |
| TDR | 88,000,801 | 88,000,821 | CCTTGCTCCCACTCAATCTTC |
PCR Primers
| Primer | Sequence |
| SU | TTCCTTTGCTGAGAAAGTAGTGC |
| SD | CAGGACCTTCAAGGAGGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
