16147 Vma21 Status: ES cell colonies screened / QC no positives
| Gene | Vma21 |
| Chromosome | X |
| Deletion Start | 71,821,117 |
| Deletion End | 71,816,891 |
| Deletion Size | 4,227 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,817,052 | 71,817,076 | GCAGGCTGATGAAGAGGTAAAGTAG |
| TUP | 71,817,077 | 71,817,101 | TTTGGAGCCTGCAGCTTCTGAGTGG |
| TUR | 71,817,139 | 71,817,159 | GACTGCAAAGCAGCCAAACAG |
| TDF | 71,820,290 | 71,820,314 | GGCCAATGACAGTTTGTATGCTTTG |
| TDP | 71,820,320 | 71,820,348 | CTGGGTGTGGTGCTGTATGCCTGTAATAC |
| TDR | 71,820,356 | 71,820,375 | GGGTCTCATGTAGCCCAGTC |
PCR Primers
| Primer | Sequence |
| SU | ATGCTGCGCGGAAAGTCC |
| SD | CCTCACTGACTTAAAAGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
