16151 Zfp711 Status: ES cell colonies screened / QC no positives
| Gene | Zfp711 |
| Chromosome | X |
| Deletion Start | 112,633,642 |
| Deletion End | 112,604,297 |
| Deletion Size | 29,346 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,604,958 | 112,604,977 | GGGCCAGGGTTATGGATTTC |
| TUP | 112,604,978 | 112,605,005 | TGTGAACTGTCTGACATGGGTGCTTAGA |
| TUR | 112,605,017 | 112,605,040 | GCTCAGTCGATAAGAATGGAGGAC |
| TDF | 112,632,680 | 112,632,701 | CAAGGAACCGAAGATGCACAAG |
| TDP | 112,632,702 | 112,632,730 | TGCAAGTACTGTGATTACGAAACTGCCGA |
| TDR | 112,632,750 | 112,632,771 | TGCTATGAACAGCCAGCAAATG |
PCR Primers
| Primer | Sequence |
| SU | GGCTGATCTGGTTCTGGAAG |
| SD | AAGTGACTGATGGATGACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
